In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococcal c2 phage diffusion and reaction through model biofilms using in situ ...
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Oct 30, 2009 · For this reason, indirect biophysical approaches, such as fluorescence correlation spectroscopy (FCS), were introduced to probe molecular dynamics in live cells (Kim et al., 2007). FCS analyzes fluorescence signals from a very small observation volume (∼10 −15 L) and determines local concentrations and diffusion coefficients of ...
Conformational subpopulations, internal dynamics and activity of single biomolecules, parameters that have so far been hidden in large ensemble averages, are now being unveiled. Herein, we review a particular attractive solution‐based single molecule technique, fluorescence correlation spectroscopy (FCS).
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Fluorescence correlation spectroscopy (FCS) was created to measure these molecular dynamics; it is sensitive to fluctuations in fluorescence intensity observed from a small open volume element (on the order of 10 −15 liter) containing only a few molecules. Fluctuations may be due to molecules diffusing in and out of the volume or to chemical transitions between fluorescent and nonfluorescent states.
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Investigation of the fluctuation dynamics of DNA solutions by laser correlation spectroscopy November 1992 · Bulletin of the Lebedev Physics Institute K.V. Grigor'ev
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Jul 01, 2015 · Unlike electron spectroscopy and microscopy techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) that require vacuum for their operation, TERS can be used in ambient environment and it is well-suited for the investigation of samples in aqueous mediums [14, 15].
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The Journal of Physical Chemistry B 2020, 124, 47, 10787-10801 (B: Liquids, Chemical and Dynamical Processes in Solution, Spectroscopy in Solution) Publication Date (Web) : November 16, 2020 Abstract
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1 March 2004 Fluorescence correlation spectroscopy investigation of a GFP mutant-enhanced cyan fluorescent protein and its tubulin fusion in living cells with two-photon excitation Zifu Wang , Jagesh V. Shah , Zhongping Chen , Chung-Ho Sun , Michael W. Berns
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Oct 01, 2011 · Fluorescence correlation spectroscopy (FCS) is a minimally invasive real-time fluorescence technique capable of detecting single molecules in vitro and in situ.By recording and correlating the fluctuations in fluorescence intensity measurements, it is possible to obtain information on molecular mobility and diffusion, hydrodynamic radii, local concentrations and photochemical and photophysical ...
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Jan 03, 2011 · Merging the features of image correlation spectroscopy and FCS, raster image correlation spectroscopy (RICS) provides a easy to implement alternative to determine dynamics and concentrations from laser scanning microscopy images [101, 102]. The sample is raster scanned and the pixels within the image are correlated.
"Studying Ultrafast Spectral Diffusion and Correlation Dynamics using Two-Dimensional Electronic Spectroscopy" by Howe-Siang TAN (Nanyang Technological University, Singapore) 15:55hrs - 16:15hrs
Molecular diffusion and transport processes are fundamental in physical, chemical, and biological systems. Current approaches to measuring molecular transport in cells and tissues based on perturbation methods, e.g., fluorescence recovery after photobleaching, are invasive; single-point fluctuation correlation methods are local; and single-particle tracking requires the observation of isolated ...
Nucleosomes and chromatin control eukaryotic genome accessibility and thereby regulate DNA processes, including transcription, replication, and repair. Conformational dynamics within the nucleosome and chromatin structure play a key role in this regulatory function. Structural fluctuations continuously expose internal DNA sequences and nucleosome surfaces, thereby providing transient access ...
Correlation analysis of the fluorescence fluctuations yields kinetic information about the dynamic processes that cause the changes in the fluorescent signal. The research group uses these concepts to investigate the dynamics, structure and kinetics of nucleoprotein assemblies.